This isn't so much a theory as it is drawing a parallel between the Sharingan and blood. But before that...
This is post no #100!! yeah...took me a year...
Anyway, on with the theory. To me, it seems as if the Sharingan has a symbolic connection to blood. Consider:
- the Sharingan is red as blood.
- to activate Mangekyou, one has to kill their best friend, and subsequently take their brother's eyes to make it permanent.
- the ability itself is passed down by blood.
considering the above, it would seem that basically, more blood = stronger Sharingan. But for me there are two sides to this coin:
1) spilling blood
2) blood ties
Let's start with the first. Spilling blood. This means that to bring the Sharingan to the next level, one has to spill blood. Mostly, it's correct, because to even get three dots in both eyes, you need experience using the Sharingan in battle, and that might involve spilling blood. However, we already know that you can train the Sharingan like anything else, so at this stage it isn't entirely necessary.
To get MS, however, one must spill the blood of one's best friend. I suppose that the idea is that a friend is even closer to the person wielding the Sharingan, and so that blood is much more significant than the blood of others.
To go even further, to make it permanent, one needs to sever an even more important bond; a brother. Basically, one later has to take one's brother's eyes, to make the power everlasting. That's it for spilling blood.
Now let's go to blood ties. This is something somewhat different on the above, since it means building ties instead of breaking them. However, so far, the only thing I can seem to find as proof is Kakashi and Obito. Now, everyone focused on Kakashi getting his MS something like 10-20 years later. We all wonder how he got it. Possibly, because all his friends (including his best friend) are dead, he might have somehow turned that to his advantage. Very possible, but my take is that it was the act of building ties and bonds that gave rise to Kakashi's Sharingan.
Consider:
- Obito activated his Sharingan while trying to protect his comrades.
- Sasuke does the same (to protect Naruto from Haku).
Meaning, rather than having the threat of danger, they activated the Sharingan to protect their friends. Later, as we all know, Obito saved Kakashi once more, this time from a falling boulder. This is where I believe the significance lies. By saving Kakashi, and not letting him get crushed, he somehow awakened the potential of MS, though a different form.
Now let me go off-topic a little and explain the main point of my theory. There are two types of MS: the conventional "dark" MS, and the repressed "light" MS. Once upon a time, before Madara came along, the Sharingan was probably considered (is still probably is, until the massacre) something that highlights the bond between the Uchiha. To gain a more powerful Sharingan, one might have to expand upon that bond, and gained the Light MS.
Then Madara and his brother came along, and found the "dark side" to gaining MS, that is, severing those bonds instead. By doing this, the blood on their hands drives them toward the darkness, and activates the MS. It is also my opinion that by doing this, gaining the Dark MS, one's eyes shall also be engulfed in the subsequent darkness that follows. The only was to return to the light, was, to steal the light of another's eyes. The eyes of their brother. By doing so, they complete the process, and complete their Dark MS, as well as gain immortality (a typical "dark side" achievement).
After years of Uchiha killing off their friends, the Dark gene in the Sharingan became more dominant, and the Light gene became weaker. And this brings us back to Kakashi and Obito. By saving Kakashi, he inadvertently re-awakened a little of the Light gene, and allowedf access to the Light MS. Instead of spilling blood on his hands, he strengthened the blood between himself and his friend. This could explain why, years later, Kakashi managed to finally manifest his MS, after all his hard work, and why it has different techniques.
One might also argue that Kakashi's eyes are deteriorating, as he hinted to Itachi. However, he might just have gotten access to the secret Uchiha meeting room, and found all the information he needed there.
I also think that to kill one's friend is not entirely necessary, just the will and dark heart to do so. The act of killing one's friend is just the more concrete way to wrench one's heart into darkness, and it just helps with the split. It is also the same way that the Light MS could be obtained. The act of total and willing sacrifice to save one's friend is needed, even if the person does not die doing so.
(will continue later)...


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